Integrin beta 3 antibody (pTyr773)

Catalog No. ABIN7139067

The Rabbit Polyclonal anti-Integrin beta 3 antibody has been validated for WB, IHC and ELISA. It is suitable to detect Integrin beta 3 in samples from Human.

Quick Overview for Integrin beta 3 antibody (pTyr773) (ABIN7139067)

Target: Integrin beta 3 (ITGB3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Integrin beta 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Integrin beta 3 Antibody

Binding Specificity: pTyr773

Cross-Reactivity: Human, Mouse

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of tyrosine 773 (P-L-Y(p)-K-E) derived from Human Integrin b3.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000, IHC:1:50-1:100,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for Integrin beta 3

Target: Integrin beta 3 (ITGB3)

Alternative Name: ITGB3

Background:

Background: Integrin a-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin a-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins a-IIb/beta-3 and a-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin a-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin a-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Chandhoke SK, et al. (2004) J Cell Sci.117 (Pt 8): 1431-1441.
Datta A, et al. (2002) J Biol Chem.277 (6): 3943-3949.
Law DA, et al. (1996) J Biol Chem.271 (18): 10811-10815.

Aliases: BDPLT16 antibody, BDPLT2 antibody, CD 61 antibody, CD61 antibody, CD61 antigen antibody, GP3A antibody, GPIIIa antibody, GT antibody, HPA 1 antibody, HPA 4 antibody, Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) antibody, Integrin beta chain beta 3 antibody, Integrin beta-3 antibody, ITB3_HUMAN antibody, ITG B3 antibody, ITGB 3 antibody, ITGB3 antibody, NAIT antibody, platelet fibrinogen receptor antibody, Platelet fibrinogen receptor beta subunit antibody, Platelet fibrinogen receptor, beta subunit antibody, Platelet glycoprotein IIIa antibody, Platelet glycoprotein IIIa precursor antibody, Platelet membrane glycoprotein IIIa antibody, PTP antibody

UniProt: P05106

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex