Cofilin antibody (pTyr88)

Catalog No. ABIN7139084

This Rabbit Polyclonal antibody specifically detects Cofilin in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for Cofilin antibody (pTyr88) (ABIN7139084)

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cofilin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Cofilin Antibody

Binding Specificity: pTyr88

Purpose: Phospho-CFL1/CFL2 (Tyr88) Antibody

Cross-Reactivity: Mouse

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of tyrosine 88 (A-T-Y(p)-E-T) derived from Human coflin1/cofilin2.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Alternative Name: CFL1

Background:

Background: Controls reversibly actin polymerization and depolymerization in a pH -sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.

Carlier, M. et al. (1999) J. Biol. Chem. 274, 33827-33830.
Arber, S. et al. (1998) Nature 393, 805-809.
Yang, N. et al. (1998) Nature 393, 809-812.

Aliases: CFL1/CFL2

UniProt: P23528, Q9Y281

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events