MLH1 antibody

Catalog No. ABIN7140335

This Mouse Monoclonal antibody specifically detects MLH1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for MLH1 antibody (ABIN7140335)

Target: MLH1 (MutL Homolog 1 (MLH1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MLH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MLH1 Antibody

Cross-Reactivity: Human

Purification: Affinity purified

Immunogen: Purified recombinant human MLH1 protein fragments expressed in E.coli.

Isotype: IgG2b

Application Details

Application Notes: Recommended dilution:WB:1:500-1:5000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified mouse monoclonal in PBS( pH 7.4) containing with 0.02 % sodium azide,0.1 mg/mLBSA and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for MLH1

Target: MLH1 (MutL Homolog 1 (MLH1))

Alternative Name: MLH1

Background:

Background: Heterodimerizes with PMS2 to form MutL alpha,a component of the post-replicative DNA mismatch repair system (MMR).DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch,then MutL alpha is recruited to the heteroduplex.Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2.It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch.DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected.MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III,suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR.Also implicated in DNA damage signaling,a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.

Aliases: COCA 2, COCA2, DNA mismatch repair protein Mlh1, FCC 2, FCC2, hMLH 1, hMLH1, HNPCC 2, HNPCC, HNPCC2, MGC5172, MLH 1, MLH1, MLH1_HUMAN, MutL homolog 1 (E. coli), MutL homolog 1, MutL homolog 1 colon cancer nonpolyposis type 2,

UniProt: P40692

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response