LC3B antibody (AA 1-116) (HRP)

Catalog No. ABIN7140915

The Rabbit Polyclonal anti-LC3B antibody has been validated for ELISA. It is suitable to detect LC3B in samples from Candida sp..

Quick Overview for LC3B antibody (AA 1-116) (HRP) (ABIN7140915)

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Reactivity: Candida sp.

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LC3B antibody is conjugated to HRP

Application: ELISA

Product Details anti-LC3B Antibody (HRP)

Binding Specificity: AA 1-116

Cross-Reactivity: Candida sp.

Purification: >95%, Protein G purified

Immunogen: Recombinant Candida glabrata Autophagy-related protein 8 protein (1-116AA)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for LC3B

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Alternative Name: ATG8

Background:

Background: Ubiquitin-like modifier involved in cytoplasm to vacuole transport (Cvt) vesicles and autophagosomes formation. With ATG4, mediates the delivery of the vesicles and autophagosomes to the vacuole via the microtubule cytoskeleton. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Participates also in membrane fusion events that take place in the early secretory pathway. Also involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress. The ATG8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy.

Aliases: ATG8 antibody, AUT7 antibody, CAGL0A04675gAutophagy-related protein 8 antibody, Autophagy-related ubiquitin-like modifier ATG8 antibody

UniProt: Q6FXR8

Pathways: Autophagy