GAPDH antibody (AA 2-333) (Biotin)

Catalog No. ABIN7141110

The Rabbit Polyclonal anti-GAPDH antibody has been validated for ELISA. It is suitable to detect GAPDH in samples from Chinese Hamster.

Quick Overview for GAPDH antibody (AA 2-333) (Biotin) (ABIN7141110)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Chinese Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAPDH antibody is conjugated to Biotin

Application: ELISA

Product Details anti-GAPDH Antibody (Biotin)

Binding Specificity: AA 2-333

Cross-Reactivity: Chinese Hamster

Purification: >95%, Protein G purified

Immunogen: Recombinant Cricetulus griseus Glyceraldehyde-3-phosphate dehydrogenase protein (2-333AA)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background:

Background: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3\'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (By similarity).

Aliases: GAPDH antibody, GAPDGlyceraldehyde-3-phosphate dehydrogenase antibody, GAPDH antibody, EC 1.2.1.12 antibody, Peptidyl-cysteine S-nitrosylase GAPDH antibody, EC 2.6.99.- antibody

UniProt: P17244