MAPK7 antibody (pSer731, pThr733) (Cy3)

Catalog No. ABIN710279

The Rabbit Polyclonal anti-MAPK7 antibody has been validated for WB, IF (cc) and IF (p). It is suitable to detect MAPK7 in samples from Human.

Quick Overview for MAPK7 antibody (pSer731, pThr733) (Cy3) (ABIN710279)

Target: MAPK7 (Mitogen-Activated Protein Kinase 7 (MAPK7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPK7 antibody is conjugated to Cy3

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-MAPK7 Antibody (Cy3)

Binding Specificity: pSer731, pThr733

Specificity: Recognizes Ser721+Thr723 in mice and rats

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human ERK5 around the phosphorylation sites of Ser731+Thr733

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MAPK7

Target: MAPK7 (Mitogen-Activated Protein Kinase 7 (MAPK7))

Alternative Name: ERK5+

Background:

Synonyms: BMK1, ERK4, ERK5, PRKM7, Mitogen-activated protein kinase 7, MAP kinase 7, MAPK 7, Big MAP kinase 1, BMK-1, Extracellular signal-regulated kinase 5, ERK-5, MAPK7

Background: Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction.

Gene ID: 5598

UniProt: Q13164

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, cAMP Metabolic Process, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling