Aurora Kinase B antibody (AA 1-255) (Biotin)

Catalog No. ABIN7145080

This anti-Aurora Kinase B antibody is a Rabbit Polyclonal antibody detecting Aurora Kinase B in ELISA. Suitable for Human.

Quick Overview for Aurora Kinase B antibody (AA 1-255) (Biotin) (ABIN7145080)

Target: Aurora Kinase B (AURKB)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aurora Kinase B antibody is conjugated to Biotin

Application: ELISA

Product Details anti-Aurora Kinase B Antibody (Biotin)

Binding Specificity: AA 1-255

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Aurora kinase B protein (1-255AA)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Alternative Name: AURKB

Background:

Background: Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis (PubMed:22422861, PubMed:24814515). AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPT1, VIM/vimentin, GSG2/Haspin, and histone H3. A positive feedback loop involving GSG2 and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at \'Ser-10\' and \'Ser-28\' during mitosis (H3S10ph and H3S28ph, respectively). A positive feedback between GSG2 and AURKB contributes to CPC localization. AURKB is also required for kinetochore localization of BUB1 and SGOL1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity. Key regulator of active promoters in resting B- and T-lymphocytes: acts by mediating phosphorylation of H3S28ph at active promoters in resting B-cells, inhibiting RNF2/RING1B-mediated ubiquitination of histone H2A and enhancing binding and activity of the USP16 deubiquitinase at transcribed genes.

Aliases: AIK2 antibody, AIM-1 antibody, AIM1 antibody, ARK-2 antibody, ARK2 antibody, AurB antibody, AURKB antibody, AURKB_HUMAN antibody, Aurora 1 antibody, Aurora and Ipl1 like midbody associated protein 1 antibody, Aurora kinase B antibody, Aurora related kinase 2 antibody, Aurora- and Ipl1-like midbody-associated protein 1 antibody, Aurora-B antibody, Aurora-related kinase 2 antibody, Aurora/IPL1 related kinase 2 antibody, Aurora/IPL1-related kinase 2 antibody, IPL1 antibody, PPP1R48 antibody, Protein phosphatase 1 regulatory subunit 48 antibody, Serine/theronine kinase 12 antibody, Serine/threonine protein kinase 12 antibody, Serine/threonine-protein kinase 12 antibody, Serine/threonine-protein kinase aurora-B antibody, STK-1 antibody, STK1 antibody, STK12 antibody, STK5 antibody

UniProt: Q96GD4

Pathways: TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades