MAP1LC3A antibody (pSer12)

Catalog No. ABIN710591

The Rabbit Polyclonal anti-MAP1LC3A antibody has been validated for ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect MAP1LC3A in samples from Rat.

Quick Overview for MAP1LC3A antibody (pSer12) (ABIN710591)

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP1LC3A antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MAP1LC3A Antibody

Binding Specificity: pSer12

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human MAP1LC3A around the phosphorylation site of Ser12

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for MAP1LC3A

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Alternative Name: MAP1LC3A

Background:

Synonyms: MAP1LC3Aphospho S12, ATG8E, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modier LC3 A, LC3, LC3A, MAP1 light chain 3 like protein 1, MAP1 light chain 3-like protein 1, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, MAP1ALC3, MAP1BLC3, Map1lc3a, Microtubule associated proteins 1A/1B light chain 3, Microtubule-associated protein 1 light chain 3 alpha, Microtubule-associated proteins 1A/1B light chain 3A, MLP3A_HUMAN.

Background: MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis.

Gene ID: 84557

Pathways: Autophagy