DAP Kinase 1 antibody (AA 1141-1430)

Catalog No. ABIN7149553

This anti-DAP Kinase 1 antibody is a Rabbit Polyclonal antibody detecting DAP Kinase 1 in ELISA. Suitable for Human.

Quick Overview for DAP Kinase 1 antibody (AA 1141-1430) (ABIN7149553)

Target: DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAP Kinase 1 antibody is un-conjugated

Application: ELISA

Product Details anti-DAP Kinase 1 Antibody

Binding Specificity: AA 1141-1430

Cross-Reactivity: Human

Purification: Antigen Affinity Purified

Immunogen: Recombinant Human Death-associated protein kinase 1 protein (1141-1430AA)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for DAP Kinase 1

Target: DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))

Alternative Name: DAPK1

Background:

Background: Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca2+ influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition. Isoform 2 cannot induce apoptosis but can induce membrane blebbing.

Aliases: DAK1 antibody, DAP K1 antibody, DAP kinase 1 antibody, DAPK 1 antibody, DAPK antibody, DAPK1 antibody, DAPK1_HUMAN antibody, Death Associated Protein Kinase 1 antibody, Death-associated protein kinase 1 antibody, DKFZp781I035 antibody

UniProt: P53355

Pathways: MAPK Signaling, Interferon-gamma Pathway