CDT2/RAMP antibody (AA 393-550) (Biotin)

Catalog No. ABIN7149692

This Rabbit Polyclonal antibody specifically detects CDT2/RAMP in ELISA. It exhibits reactivity toward Human.

Quick Overview for CDT2/RAMP antibody (AA 393-550) (Biotin) (ABIN7149692)

Target: CDT2/RAMP (DTL) (Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDT2/RAMP antibody is conjugated to Biotin

Application: ELISA

Product Details anti-CDT2/RAMP Antibody (Biotin)

Binding Specificity: AA 393-550

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Denticleless protein homolog protein (393-550AA)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for CDT2/RAMP

Target: CDT2/RAMP (DTL) (Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL))

Alternative Name: DTL

Background:

Background: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1, CDKN1A/p21(CIP1), FBXO18/FBH1 and KMT5A (PubMed:16861906, PubMed:16949367, PubMed:16964240, PubMed:17085480, PubMed:18703516, PubMed:18794347, PubMed:18794348, PubMed:19332548, PubMed:20129063, PubMed:23478441, PubMed:23478445, PubMed:23677613). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication (PubMed:16861906, PubMed:16949367, PubMed:17085480). CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing (PubMed:18794348, PubMed:19332548). KMT5A degradation is also important for a proper regulation of mechanisms such as TGF-beta signaling, cell cycle progression, DNA repair and cell migration (PubMed:23478445). Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the \'K+4\' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the \'Lys-164\' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis (PubMed:20129063, PubMed:23478441, PubMed:23478445, PubMed:23677613).

Aliases: Lethal(2) denticleless protein homolog antibody, CDW1 antibody, DCAF2 antibody, DDB1 and CUL4 associated factor 2 antibody, Ddb1- and Cul4-associated factor 2 antibody, Denticleless homolog antibody, Denticleless homolog (Drosophila) antibody, Denticleless protein homolog antibody, Dtl antibody, DTL_HUMAN antibody, L2DTL antibody, Lethal(2) denticleless protein homolog antibody, RA regulated nuclear matrix associated protein antibody, RAMP antibody, Retinoic acid regulated nuclear matrix associated protein antibody, Retinoic acid-regulated nuclear matrix-associated protein antibody

UniProt: Q9NZJ0