DPPA3 antibody (AA 1-159) (Biotin)

Catalog No. ABIN7149821

This Rabbit Polyclonal antibody specifically detects DPPA3 in ELISA. It exhibits reactivity toward Human.

Quick Overview for DPPA3 antibody (AA 1-159) (Biotin) (ABIN7149821)

Target: DPPA3 (Developmental Pluripotency Associated 3 (DPPA3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DPPA3 antibody is conjugated to Biotin

Application: ELISA

Product Details anti-DPPA3 Antibody (Biotin)

Binding Specificity: AA 1-159

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Developmental pluripotency-associated protein 3 protein (1-159AA)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for DPPA3

Target: DPPA3 (Developmental Pluripotency Associated 3 (DPPA3))

Alternative Name: DPPA3

Background:

Background: Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at \'Lys-9\' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis (By similarity).

Aliases: Compaction associated protein 1 antibody, Developmental pluripotency associated 3 antibody, Developmental pluripotency associated protein 3 antibody, Developmental pluripotency-associated protein 3 antibody, Dppa3 antibody, DPPA3_HUMAN antibody, PGC7 antibody, Stella related protein antibody, Stella-related protein antibody

UniProt: Q6W0C5