GRP78 antibody (AA 25-293) (HRP)

Catalog No. ABIN7151666

This Rabbit Polyclonal antibody specifically detects GRP78 in ELISA. It exhibits reactivity toward Human.

Quick Overview for GRP78 antibody (AA 25-293) (HRP) (ABIN7151666)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRP78 antibody is conjugated to HRP

Application: ELISA

Product Details anti-GRP78 Antibody (HRP)

Binding Specificity: AA 25-293

Purpose: HSPA5 Antibody, HRP conjugated

Purification: Protein G purified

Purity: >95 %

Immunogen: Recombinant Human Endoplasmic reticulum chaperone BiP protein (25-293AA)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Alternative Name: HSPA5

Background:

Background: Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen (PubMed:2294010, PubMed:23769672, PubMed:23990668, PubMed:28332555). Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate (By similarity). Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) (PubMed:1550958, PubMed:19538957). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1 (By similarity). Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1 (By similarity). Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER). May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating.

Aliases: 78 kDa glucose regulated protein antibody, 78 kDa glucose-regulated protein antibody, AL022860 antibody, AU019543 antibody, BIP antibody, D2Wsu141e antibody, D2Wsu17e antibody, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody, Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody, Epididymis secretory sperm binding protein Li 89n antibody, FLJ26106 antibody, Glucose Regulated Protein 78 antibody, Glucose Regulated Protein 78 kDa antibody, GRP 78 antibody, GRP-78 antibody, GRP78 antibody, GRP78_HUMAN antibody, Heat shock 70 kDa protein 5 antibody, Heat Shock 70 kDa Protein 5 antibody, Heat shock protein family A (Hsp70) member 5 antibody, HEL S 89n antibody, Hsce70 antibody, HSPA 5 antibody, HSPA5 antibody, Immunoglobulin Heavy Chain Binding Protein antibody, Immunoglobulin heavy chain-binding protein antibody, mBiP antibody, MIF2 antibody, Sez7 antibody

UniProt: P11021

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling