PRKAA1 antibody (pThr198)

Catalog No. ABIN711236

The Rabbit Polyclonal anti-PRKAA1 antibody has been validated for WB, ELISA, IHC (p), FACS, IF (cc), IF (p) and IHC (fro). It is suitable to detect PRKAA1 in samples from Human, Mouse, Rat and Dog.

Quick Overview for PRKAA1 antibody (pThr198) (ABIN711236)

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PRKAA1 Antibody

Binding Specificity: pThr198

Cross-Reactivity: Dog, Human, Mouse, Rat

Predicted Reactivity: Cow,Sheep,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human PRKAA1 around the phosphorylation site of Thr198

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PRKAA1

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Alternative Name: AMPK alpha-1

Background:

Synonyms: AMPK alpha 1 + AMPK alpha 2 phospho S485 + S491, PRKAA1phospho S79, 5 AMP activated protein kinase alpha 1catalytic subunit, 5 AMP activated protein kinase catalytic alpha 1 chain, 5' AMP activated protein kinase catalytic subunit alpha 1, AAPK1,acetyl CoA carboxylase kinase,AI194361 antibody AI450832 ,AL024255 ,AMP -activate kinase alpha 1 subunit,AMP-activated protein kinase, catalytic, alpha -1,AMPK 1,AMPK alpha 1 chain,AMPK antibody AMPK1, AMPKa1,AMPKalpha1,C130083N04Rik,cb116 antibody EC 2.7.11.1, HMG CoA reductase kinase,hormone sensitive lipase kinase,im:7154392 antibody kinase AMPK alpha1 ,MGC33776,MGC57364 antibody PRKAA 1,PRKAA1,Protein kinase AMP activated alpha 1 catalytic subunit, SNF1-like protein AMPK,wu:fa94C10.

Background: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq].

Molecular Weight: 63kDa

Gene ID: 5562

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect