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Hsc70 antibody (AA 2-646)

Name: Hsc70 antibody (AA 2-646)
Brand: antibodies-online
SKU: ABIN7154884
Price: 462 USD
Availability: InStock

This anti-Hsc70 antibody is a Mouse Monoclonal antibody detecting Hsc70 in WB, IHC, ELISA, IF and FACS. Suitable for Human.

Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate All lanes HSPA8 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 70~75 KDa Observed band size: 70~75 KDa Exposure time: 5 min (Hsc70 antibody (AA 2-646))

Overlay histogram showing MCF-7 cells stained with ABIN7154884 (red line). (Hsc70 antibody (AA 2-646))

FACS

Western Blot Positive WB detected in: Hela whole cell lysate at 20 μg, 10 μg, 5 μg, 2. (Hsc70 antibody (AA 2-646))

Western Blot Positive WB detected in: 20 μg hela whole cell lysate HSPA8 antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 70~75 KDa Observed band size: 70~75 KDa Exposure time: 5 min (Hsc70 antibody (AA 2-646))

IHC image of ABIN7154884 diluted at 1:220 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. (Hsc70 antibody (AA 2-646))

IHC

IHC image of ABIN7154884 diluted at 1:220 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. (Hsc70 antibody (AA 2-646))

IHC

Immunofluorescence staining of Hela cells with ABIN7154884 at 1:100, counter-stained with DAPI. (Hsc70 antibody (AA 2-646))

Immunofluorescence staining of MCF-7 cells with ABIN7154884 at 1:100, counter-stained with DAPI. (Hsc70 antibody (AA 2-646))

Immunofluorescence staining of PC-3 cells with ABIN7154884 at 1:100, counter-stained with DAPI. (Hsc70 antibody (AA 2-646))

9 images

Catalog No. ABIN7154884

Quick Overview for Hsc70 antibody (AA 2-646) (ABIN7154884)

Target

See all Hsc70 (HSPA8) Antibodies

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Reactivity

Human

Host

Mouse

Clonality

Monoclonal

Conjugate

This Hsc70 antibody is un-conjugated

Application

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Clone

6E4D2

Discover our top product HSPA8 Primary Antibody

Binding Specificity
16

7

6

5

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 2-646

Purpose

HSPA8 Monoclonal Antibody

Purification

Protein G purified

Purity

>95 %

Immunogen

Recombinant Human Heat shock cognate 71 kDa protein (2-646AA)

Isotype

IgG1
anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 1-300) antibody
HSPA8 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 2-646) antibody
HSPA8 Reactivity: Human WB, IHC, ELISA, IP, FACS Host: Mouse Monoclonal 2G8F6 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (C-Term) antibody
HSPA8 Reactivity: Human, Rat, Mouse WB, ICC, IF, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 539-569), (C-Term) antibody
HSPA8 Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16528 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 82-110), (N-Term) antibody
HSPA8 Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB17655 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) antibody
HSPA8 Reactivity: Human WB, IHC, ELISA, IP, ICC, IF, Bind, PLA, AA Host: Mouse Monoclonal 1F2-H5 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 13-31), (N-Term) antibody
HSPA8 Reactivity: Human, Rat, Mouse WB, IHC, ICC, IF, FACS, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (N-Term) antibody
HSPA8 Reactivity: Human, Rat, Mouse, Cow, Sheep, Pig, Zebrafish (Danio rerio), Goat WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 534-615) antibody
HSPA8 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 418-597) antibody
HSPA8 Reactivity: Human WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C,-80 °C

Storage Comment

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Alternative Name

HSPA8

Background

Background: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Participates in the ER-associated degradation (ERAD) quality control pathway in conjunction with J domain-containing co-chaperones and the E3 ligase CHIP
Aliases: Heat shock cognate 71 kDa protein (Heat shock 70 kDa protein 8) (Lipopolysaccharide-associated protein 1) (LAP-1) (LPS-associated protein 1), HSPA8, HSC70 HSP73 HSPA10

UniProt

P11142