IRAK4 antibody (AA 1-220)

Catalog No. ABIN7156752

The Rabbit Polyclonal anti-IRAK4 antibody has been validated for WB, ELISA and IP. It is suitable to detect IRAK4 in samples from Human.

Quick Overview for IRAK4 antibody (AA 1-220) (ABIN7156752)

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRAK4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-IRAK4 Antibody

Binding Specificity: AA 1-220

Cross-Reactivity: Human

Purification: Antigen Affinity Purified

Immunogen: Recombinant Human Interleukin-1 receptor-associated kinase 4 protein (1-220AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:500-1:2000, IP:1:200-1:2000, IP:1:200-1:2000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for IRAK4

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Alternative Name: IRAK4

Background:

Background: Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.

Aliases: IL-1 receptor-associated kinase 4 antibody, Interleukin 1 receptor associated kinase 4 mutant form 1 antibody, Interleukin-1 receptor-associated kinase 4 antibody, Interleukin1 receptor associated kinase 4 antibody, IPD1 antibody, IRAK 4 antibody, IRAK-4 antibody, IRAK4 antibody, IRAK4 mutated form 1 antibody, IRAK4_HUMAN antibody, LOC 51135 antibody, NY REN 64 antibody, NY REN 64 antigen antibody, NY-REN-64 antibody, REN64 antibody, Renal carcinoma antigen NY-REN-64 antibody

UniProt: Q9NWZ3

Pathways: NF-kappaB Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades