SMAD3 antibody (AA 1-230)

Catalog No. ABIN7160232

The Rabbit Polyclonal anti-SMAD3 antibody has been validated for WB, ELISA, IHC, IF and IP. It is suitable to detect SMAD3 in samples from Human.

Quick Overview for SMAD3 antibody (AA 1-230) (ABIN7160232)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-SMAD3 Antibody

Binding Specificity: AA 1-230

Cross-Reactivity: Human

Purification: Antigen Affinity Purified

Immunogen: Recombinant Human Mothers against decapentaplegic homolog 3 protein (1-230AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:500, IF:1:50-1:200, IP:1:200-1:2000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name: SMAD3

Background:

Background: Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Aliases: DKFZP586N0721 antibody, DKFZp686J10186 antibody, hMAD 3 antibody, hMAD-3 antibody, hSMAD3 antibody, HSPC193 antibody, HST17436 antibody, JV15 2 antibody, JV15-2 antibody, JV152 antibody, LDS1C antibody, LDS3 antibody, MAD (mothers against decapentaplegic Drosophila) homolog 3 antibody, MAD homolog 3 antibody, Mad homolog JV15 2 antibody, Mad protein homolog antibody, MAD, mothers against decapentaplegic homolog 3 antibody, Mad3 antibody, MADH 3 antibody, MADH3 antibody, MGC60396 antibody, Mothers against decapentaplegic homolog 3 antibody, Mothers against DPP homolog 3 antibody, SMA and MAD related protein 3 antibody, SMAD 3 antibody, SMAD antibody, SMAD family member 3 antibody, SMAD, mothers against DPP homolog 3 antibody, Smad3 antibody, SMAD3_HUMAN antibody

UniProt: P84022

Pathways: Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy