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Nestin antibody

This anti-Nestin antibody is a Mouse Monoclonal antibody detecting Nestin in WB, IHC, ELISA, IF and FACS. Suitable for Human.

Western Blot Positive WB detected in: U251 whole cell lysate, SH-SY5Y whole cell lysate All lanes: NES antibody at 3 μg/mL Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 178 kDa Observed band size: 260 kDa (Nestin antibody)

Overlay histogram showing Hela cells stained with ABIN7161178 (red line). (Nestin antibody)

Immunohistochemistry of paraffin-embedded human tonsil tissue using ABIN7161178 at dilution of 1:100 (Nestin antibody)

Immunohistochemistry of paraffin-embedded human kidney tissue using ABIN7161178 at dilution of 1:100 (Nestin antibody)

Immunofluorescent analysis of Hela cells using ABIN7161178 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). (Nestin antibody)

Immunofluorescent analysis of PC-3 cells using ABIN7161178 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). (Nestin antibody)

Immunofluorescent analysis of U251 cells using ABIN7161178 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). (Nestin antibody)

Immunohistochemistry of paraffin-embedded human melanoma using ABIN7161178 at dilution of 1:100 (Nestin antibody)

Overlay histogram showing U251 cells stained with ABIN7161178 (red line). (Nestin antibody)

9 images

Catalog No. ABIN7161178

Quick Overview for Nestin antibody (ABIN7161178)

Target

See all Nestin (NES) Antibodies

Nestin (NES)

Reactivity

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39
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4
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1

Human

Host

Mouse

Clonality

Monoclonal

Conjugate

65
8
5
5
4
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2
2
2
2
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2
1
1
1
1
1
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1
1
1
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1
1
1
1
1
1

This Nestin antibody is un-conjugated

Application

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54
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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Clone

2C2F7

Discover our top product NES Primary Antibody

Cross-Reactivity

Human

Purification

Protein G purified

Immunogen

Recombinant Human Nestin protein

Isotype

IgG1, IgG1 kappa
anti-Nestin (NES) (AA 1389-1416) antibody
NES Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16253 unconjugated

anti-Nestin (NES) (AA 178-399) antibody
NES Reactivity: Human WB, IHC, ICC, IP Host: Mouse Monoclonal D10 unconjugated

anti-Nestin (NES) (AA 252-290), (N-Term) antibody
NES Reactivity: Human, Mouse, Rat WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Nestin (NES) antibody
NES Reactivity: Mouse IHC, ICC Host: Rat Monoclonal 7A3 unconjugated

anti-Nestin (NES) (AA 698-844) antibody
NES Reactivity: Human IHC (f) Host: Mouse Monoclonal NES-2911 unconjugated

anti-Nestin (NES) (AA 1484-1500) antibody
NES Reactivity: Human, Mouse WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

anti-Nestin (NES) (AA 1389-1416) antibody
NES Reactivity: Human WB, IHC, ELISA, IF, FACS Host: Rabbit Polyclonal unconjugated

anti-Nestin (NES) antibody (FITC)
NES Reactivity: Human ICC, IF Host: Mouse Monoclonal 10C2 FITC

anti-Nestin (NES) (AA 1392-1621) antibody
NES Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

anti-Nestin (NES) (AA 681-750) antibody
NES Reactivity: Human, Mouse, Rat ELISA, FACS, IHC (p), IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

Application Notes

Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:500, IF:1:50-1:200,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C,-80 °C

Storage Comment

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

Nestin (NES)

Alternative Name

NES

Background

Background: Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Aliases: Nestin, NES, Nbla00170

UniProt

P48681

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