TSC1 antibody (pSer505)

Catalog No. ABIN711971

The Rabbit Polyclonal anti-TSC1 antibody has been validated for ELISA, WB, IF (cc), IF (p), IHC (p) and IHC (fro). It is suitable to detect TSC1 in samples from Human, Mouse and Sheep. There is 1 publication available.

Quick Overview for TSC1 antibody (pSer505) (ABIN711971)

Target: TSC1 (Tuberous Sclerosis 1 (TSC1))

Reactivity: Human, Mouse, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TSC1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-TSC1 Antibody

Binding Specificity: pSer505

Cross-Reactivity: Human, Mouse, Sheep

Predicted Reactivity: Rat,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human TSC1 around the phosphorylation site of Ser505 [FD(p-S)PF]

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-TSC1 antibody (ABIN711971)

Cao, Wei, Liu, Wang, Wu, Xie, Capellini, Zhang, Zhao, Li, Zhong, Wang, Lu, Liu, Zhang, Du, Zhang, Du: "DNA methylation Landscape of body size variation in sheep." in: Scientific reports, Vol. 5, pp. 13950, (2016) (PubMed).

Target Details for TSC1

Target: TSC1 (Tuberous Sclerosis 1 (TSC1))

Alternative Name: TSC1

Background:

Synonyms: TSC1phospho S505, LAM, TSC1, Tuberous sclerosis 1 protein.

Background: Hamartin, or TSC1, is a suspected tumor suppressor implicated in the disease tuberous sclerosis 1. It is a negative regulator of cell division controlling the transition from G0/G1 to S phase, and it seems to act through the phosphatidylinositol 3 kinase/Akt pathway. TSC1 interacts with tuberin m(TSC2), which is thought to be a GAP (GTPase Activating Protein) for the Rap1 and Rab5 small G Proteins. The Hamartin/Tuberin complex has been shown to inhibit mTor. Hamartin has also been shown to interact with ERM (Ezrin-Radixin-Moesin) proteins and with F-actin, suggesting a role for TSC proteins in modulation of cell adhesion and morphology.

Gene ID: 7248

Pathways: RTK Signaling, AMPK Signaling, Regulation of Cell Size, Tube Formation