PARP1 antibody (AA 324-541)

Catalog No. ABIN7163739

The Rabbit Polyclonal anti-PARP1 antibody has been validated for WB, ELISA, IHC and IF. It is suitable to detect PARP1 in samples from Human.

Quick Overview for PARP1 antibody (AA 324-541) (ABIN7163739)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PARP1 Antibody

Binding Specificity: AA 324-541

Cross-Reactivity: Human

Purification: Antigen Affinity Purified

Immunogen: Recombinant Human Poly [ADP-ribose] polymerase 1 protein (324-541aa)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:1000-1:5000, IHC:1:100-1:500, IF:1:100-1:500,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: PARP1

Background:

Background: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (PubMed:17177976, PubMed:18172500, PubMed:19344625, PubMed:19661379, PubMed:23230272). Mediates the poly(ADP-ribosyl)ation of APLF and CHFR (PubMed:17396150). Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production (PubMed:17177976). Required for PARP9 and DTX3L recruitment to DNA damage sites (PubMed:23230272). PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (PubMed:23230272). Mediates serine ADP-ribosylation of target proteins following interaction with HPF1, HPF1 conferring serine specificity (PubMed:28190768). Mediates the poly(ADP-ribosyl)ation of histones in a HPF1-dependent manner (PubMed:27067600). Involved in the synthesis of ATP in the nucleus, together with NMNAT1, PARG and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257).

Aliases: ADP ribosyltransferase (NAD+, poly (ADP ribose) polymerase) antibody, ADP ribosyltransferase antibody, ADP ribosyltransferase diphtheria toxin like 1 antibody, ADP ribosyltransferase NAD(+) antibody, ADPRT 1 antibody, ADPRT antibody, ADPRT1 antibody, ARTD1 antibody, msPARP antibody, NAD(+) ADP ribosyltransferase 1 antibody, NAD(+) ADP-ribosyltransferase 1 antibody, pADPRT 1 antibody, pADPRT-1 antibody, pADPRT1 antibody, PARP 1 antibody, PARP antibody, PARP-1 antibody, PARP1 antibody, PARP1_HUMAN antibody, Poly (ADP ribose) polymerase 1 antibody, poly (ADP ribose) polymerase family, member 1 antibody, Poly (ADP-ribose) polymerase 1 antibody, Poly [ADP-ribose] polymerase 1 antibody, Poly(ADP ribose) polymerase antibody, poly(ADP ribose) synthetase antibody, poly(ADP ribosyl)transferase antibody, Poly(ADP-ribosyl)transferase antibody, Poly[ADP ribose] synthetase 1 antibody, Poly[ADP-ribose] synthase 1 antibody, PPOL antibody, sPARP 1 antibody, sPARP1 antibody

UniProt: P09874

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location