PAF1/PD2 antibody (AA 1-300)

Catalog No. ABIN7168299

Product Details anti-PAF1/PD2 Antibody

Target: PAF1/PD2 (PAF1) (Paf1, RNA Polymerase II Associated Factor, Homolog (PAF1))

Binding Specificity: AA 1-300

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAF1/PD2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Cross-Reactivity: Human, Mouse

Purification: >95%, Protein G purified

Immunogen: Recombinant Human RNA polymerase II-associated factor 1 homolog protein (1-300AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:2000-1:10000, IHC:1:20-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for PAF1/PD2

Target: PAF1/PD2 (PAF1) (Paf1, RNA Polymerase II Associated Factor, Homolog (PAF1))

Alternative Name: PAF1 (PAF1 Products)

Synonyms: F23149_1 antibody, PD2 antibody, 5730511K23Rik antibody, AA675045 antibody, f23149_1 antibody, pd2 antibody, im:6906070 antibody, paf1l antibody, wu:fb80a09 antibody, paf1 antibody, RGD1306219 antibody, PAF1 homolog, Paf1/RNA polymerase II complex component antibody, Paf1, RNA polymerase II complex component antibody, PAF1 homolog, Paf1/RNA polymerase II complex component L homeolog antibody, RNA polymerase II-associated factor 1 homolog pseudogene antibody, RNA polymerase II-associated factor 1 homolog antibody, paf1, RNA polymerase II associated factor, homolog (S. cerevisiae) antibody, Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae) antibody, PAF1 antibody, Paf1 antibody, paf1 antibody, paf1.L antibody, LOC100019208 antibody, LOC100161171 antibody, LOC100639019 antibody, LOC100650306 antibody

Background:

Background: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and \\\'Ser-2\\\'- and \\\'Ser-5\\\'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1, it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 \\\'Lys-4\\\' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of \\\'Lys-120\\\' of histone H2B (H2BK120ub1), UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3\\\' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Connects PAF1C with the RNF20/40 E3 ubiquitin-protein ligase complex. Involved in polyadenylation of mRNA precursors. Has oncogenic activity in vivo and in vitro.

Aliases: F23149 1 antibody, FLJ11123 antibody, hPAF1 antibody, PAF 1 antibody, PAF1 antibody, Paf1 RNA polymerase II associated factor homolog antibody, PAF1_HUMAN antibody, Pancreatic differentiation protein 2 antibody, PD 2 antibody, PD2 antibody, PD2 protein antibody, RNA polymerase II associated factor homolog antibody, RNA polymerase II-associated factor 1 homolog antibody

UniProt: Q8N7H5

Pathways: Cellular Response to Molecule of Bacterial Origin, Stem Cell Maintenance