WNK4 antibody (AA 828-1106)

Catalog No. ABIN7169234

This Rabbit Polyclonal antibody specifically detects WNK4 in ELISA, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for WNK4 antibody (AA 828-1106) (ABIN7169234)

Target: WNK4 (WNK Lysine Deficient Protein Kinase 4 (WNK4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WNK4 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-WNK4 Antibody

Binding Specificity: AA 828-1106

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Serine/threonine-protein kinase WNK4 protein (828-1106AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for WNK4

Target: WNK4 (WNK Lysine Deficient Protein Kinase 4 (WNK4))

Alternative Name: WNK4

Background:

Background: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K(+) channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K(+) secretion to maintain integrated homeostasis. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition (By similarity).

Aliases: PHA 2B antibody, PHA2B antibody, PRKWNK 4 antibody, PRKWNK4 antibody, Protein kinase lysine deficient 4 antibody, Protein kinase lysine-deficient 4 antibody, Protein kinase with no lysine 4 antibody, Serine/threonine protein kinase WNK4 antibody, Serine/threonine-protein kinase WNK4 antibody, WNK lysine deficient protein kinase 4 antibody, Wnk4 antibody, WNK4_HUMAN antibody

UniProt: Q96J92