ROCK2 antibody (pThr249) (HRP)

Catalog No. ABIN713090

The Rabbit Polyclonal anti-ROCK2 antibody is suitable to detect ROCK2 in samples from Human. It has been validated for WB, ELISA, IHC (p) and IHC (fro).

Quick Overview for ROCK2 antibody (pThr249) (HRP) (ABIN713090)

Target: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ROCK2 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ROCK2 Antibody (HRP)

Binding Specificity: pThr249

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Pig,Horse,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human ROCK2 around the phosphorylation site of Thr249

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ROCK2

Target: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Alternative Name: ROCK2

Background:

Synonyms: ROCK2phospho T249, p164 ROCK 2, Rho associated coiled coil containing protein kinase 2, Rho associated protein kinase 2, Rho kinase 2, ROCK 2, Rock II, Rock2m, ROK alpha, ROKalpha, ROCK2_HUMAN.

Background: The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

Gene ID: 9475

Pathways: Microtubule Dynamics, WNT Signaling, Tube Formation