RPS6KA3 antibody (pSer369)

Catalog No. ABIN713126

Product Details anti-RPS6KA3 Antibody

Target: RPS6KA3 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))

Binding Specificity: pSer369

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS6KA3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human RSK2 around the phosphorylation site of Ser369 [KD(p-S)PG]

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for RPS6KA3

Target: RPS6KA3 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))

Alternative Name: RSK2 (RPS6KA3 Products)

Background:

Synonyms: RPS6KA3phospho S369, Rsk 2, MAPKAP Kinase 1b, MAP kinase activated protein kinase 1b, MAPK activated protein kinase 1b, 90 kDa ribosomal protein S6 kinase 3, HU2, HU3, Insulin stimulated protein kinase 1, ISPK1, MAPKAPK 1b, MAPKAPK1B, MRX19, p90 RSK3, p90RSK3, pp90RSK2, Ribosomal protein S6 kinase 90 kDa polypeptide 3, Ribosomal protein S6 kinase alpha 3, Ribosomal S6 kinase 2, RPS6KA3, S6 kinase 2, S6K alpha3, KS6A3_HUMAN.

Background: RSK2 or MAPKAP Kinase 1b is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains two non-identical kinase catalytic domains and phosphorylates various substrates including ribosomal protein S6 and members of the mitogen activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin Lowry syndrome.

Gene ID: 6197

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades