S100A8 antibody (AA 2-89) (FITC)

Catalog No. ABIN7177794

The Rabbit Polyclonal anti-S100A8 antibody has been validated for . It is suitable to detect S100A8 in samples from Mouse.

Quick Overview for S100A8 antibody (AA 2-89) (FITC) (ABIN7177794)

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S100A8 antibody is conjugated to FITC

Application: Please inquire

Product Details anti-S100A8 Antibody (FITC)

Binding Specificity: AA 2-89

Purpose: S100a8 Antibody, FITC conjugated

Purification: Protein G purified

Purity: >95 %

Immunogen: Recombinant Mouse Protein S100-A8 protein (2-89AA)

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for S100A8

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Alternative Name: S100a8

Background:

Background: S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn2+ which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect, regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants.

Aliases: S100a8 antibody, Caga antibody, Mrp8 antibody, Protein S100-A8 antibody, Calgranulin-A antibody, Chemotactic cytokine CP-10 antibody, Leukocyte L1 complex light chain antibody, Migration inhibitory factor-related protein 8 antibody, MRP-8 antibody, p8 antibody, Pro-inflammatory S100 cytokine antibody, S100 calcium-binding protein A8 antibody

UniProt: P27005

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins