TK1 antibody (Ser13)

Catalog No. ABIN7180098

The Rabbit Polyclonal anti-TK1 antibody has been validated for WB, ELISA, IHC and IF. It is suitable to detect TK1 in samples from Human.

Quick Overview for TK1 antibody (Ser13) (ABIN7180098)

Target: TK1 (Thymidine Kinase 1, Soluble (TK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-TK1 Antibody

Binding Specificity: Ser13

Cross-Reactivity: Human, Mouse

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized non-phosphopeptide derived from Human TK around the phosphorylation site of serine 13 (P-G-S(p)-P-S).

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for TK1

Target: TK1 (Thymidine Kinase 1, Soluble (TK1))

Alternative Name: TK1

Background:

Background: Thymidine kinases play a critical role in generating the DNA synthetic precursor deoxythymidine triphosphate (dTTP) by catalyzing the phosphotransfer of phosphate from ATP to deoxythymidine (dT) and thymidine (T) in the cell.TK1 expression and activity is regulated in a cell cycle-dependent manner, accumulating during G1-phase to peak levels in S-phase before being degraded prior to cell division. Stability, but not activity, may be regulated via phosphorylation of TK1 at Ser13 by Cdc2 and/or Cdk2, but the precise mode of regulation remains elusive. These observations indicate that TK1 might be a useful marker of cell proliferation, however, recent studies have shown that TK1 plays a more significant role in the DNA damage response.

N Singh, Mol. Cell. Biol., May 1991, 11: 2362 - 2374.
Bin Tian, Am. J. Pathol., Aug 2003, 163: 789.
RenGerolami, Cancer Res., Feb 2000, 60: 993 - 1001.
Catia Traversari, Blood, Jun 2007, 109: 4708 - 4715

Aliases: cytosolic antibody, KITH_HUMAN antibody, Thymidine kinase 1 antibody, Thymidine kinase 1 soluble antibody, Thymidine kinase 1 soluble isoform antibody, Thymidine kinase antibody, Thymidine kinase cytosolic antibody, TK 1 antibody, TK 2 antibody, TK1 antibody, Tk1a antibody, Tk1b antibody, TK2 antibody

UniProt: P04183