Topoisomerase II alpha antibody (Thr1343)

Catalog No. ABIN7180100

This Rabbit Polyclonal antibody specifically detects Topoisomerase II alpha in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Topoisomerase II alpha antibody (Thr1343) (ABIN7180100)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Topoisomerase II alpha Antibody

Binding Specificity: Thr1343

Cross-Reactivity: Human

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized non-phosphopeptide derived from Human TOP2A around the phosphorylation site of threonine 1343 (E-K-T(p)-D-D).

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:3000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: TOP2A

Background:

Background: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes.

Tsai-Pflugfelder M., Proc. Natl. Acad. Sci. U.S.A. 85:7177-7181(1988).
Wasserman R.A., Cancer Res. 53:3591-3596(1993).
Lang A.J., Gene 221:255-266(1998).

Aliases: alpha isozyme antibody, ATP hydrolyzing DNA topoisomerase II alfa antibody, DNA gyrase antibody, DNA topoisomerase (ATP hydrolyzing) antibody, DNA topoisomerase 2 alpha antibody, DNA topoisomerase 2-alpha antibody, DNA topoisomerase II 170 kD antibody, DNA topoisomerase II alpha isozyme antibody, DNA topoisomerase II antibody, DNA Topoisomerase2 antibody, TOP 2A antibody, TOP2 antibody, TOP2A antibody, TOP2A_HUMAN antibody, Topoisomerase DNA II alpha 170 kDa antibody, TP2A antibody

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases