TBP antibody (AA 1-144)

Catalog No. ABIN7193395

This Mouse Monoclonal antibody specifically detects TBP in WB, ELISA, IHC, ICC and FACS. It exhibits reactivity toward Human.

Quick Overview for TBP antibody (AA 1-144) (ABIN7193395)

Target: TBP (TATA Box Binding Protein (TBP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TBP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 7G11C8

Product Details anti-TBP Antibody

Binding Specificity: AA 1-144

Purpose: TBP Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human TBP (AA: 1-144) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Target Details for TBP

Target: TBP (TATA Box Binding Protein (TBP))

Alternative Name: TBP

Background:

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 25-42, and expansion of the number of repeats to 45-66 increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2016]

Molecular Weight: 37.7 kDa

UniProt: P20226

Pathways: WNT Signaling