Calreticulin antibody (AA 18-417)

Catalog No. ABIN7193510

The Mouse Monoclonal anti-Calreticulin antibody has been validated for WB, IHC, ELISA and FACS. It is suitable to detect Calreticulin in samples from Human, Mouse and Rat.

Quick Overview for Calreticulin antibody (AA 18-417) (ABIN7193510)

Target: Calreticulin (CALR)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calreticulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Clone: 7B3D7

Product Details anti-Calreticulin Antibody

Binding Specificity: AA 18-417

Purpose: CALR Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human CALR (AA: 18-417) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Target Details for Calreticulin

Target: Calreticulin (CALR)

Alternative Name: CALR

Background:

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.

Molecular Weight: 48.1 kDa

Gene ID: 811

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response