IKZF1 antibody (AA 401-520)

Catalog No. ABIN7193822

The Mouse Monoclonal anti-IKZF1 antibody has been validated for ELISA, FACS and ICC. It is suitable to detect IKZF1 in samples from Human.

Quick Overview for IKZF1 antibody (AA 401-520) (ABIN7193822)

Target: IKZF1 (IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IKZF1 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 1C1B6

Product Details anti-IKZF1 Antibody

Binding Specificity: AA 401-520

Purpose: IKZF1 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human IKZF1 (AA: 401-520) expressed in E. Coli.

Isotype: IgG2b

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Target Details for IKZF1

Target: IKZF1 (IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1))

Alternative Name: IKZF1

Background:

This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL).

Molecular Weight: 57.5 kDa

UniProt: Q13422

Pathways: Production of Molecular Mediator of Immune Response