CD4 antibody

Catalog No. ABIN7200645

The Mouse Monoclonal anti-CD4 antibody has been validated for FACS. It is suitable to detect CD4 in samples from Human, Rhesus Monkey and Cynomolgus.

Quick Overview for CD4 antibody (ABIN7200645)

Target: CD4

Reactivity: Human, Rhesus Monkey, Cynomolgus

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Grade: GMP Grade

Clone: SK3

Product Details anti-CD4 Antibody

Purpose: CD4 Unconjugated Antibody

Characteristics: The clone SK3, a mouse monoclonal antibody selectively binds with a 55-59kD type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. CD4 contains four extracellular Ig-like domains (D1-D4). The epitope for SK3 is located within the D3 domain of the protein, which has a structure resembling an Ig variable domain. Expression of CD4 is observed in subsets of T lymphocytes, monocytes, macrophages, and dendritic cells. Through interaction of MHC-II, CD4 facilitates cell-cell interaction, thymic differentiation, and activation of downstream signaling cascades. HIV infection of T-cells is instigated through binding of HIV to CD4. Binding of SK3 antibody blocks HIV infection through CD4 and also blocks the mixed lymphocyte reaction (MLR).

Purification: Purified

Purity: >95 %

Immunogen: Human peripheral blood T cells

Isotype: IgG1, kappa

Application Details

Application Notes: Each lot of these antibodies has been pre-titrated and tested by flow cytometric analysis of human PBMCs such that 0.5µg (unconjugated) of these products are sufficient for staining 1 million cells in a 100µl staining volume or 100µl of whole blood. It is recommended to titrate antibody reactivity empirically for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS pH 7.2, 0.1 % (w/v) BSA, 0.09 % (w/v) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CD4

Target: CD4

Alternative Name: CD4

Gene ID: 920

NCBI Accession: NM_000616

UniProt: P01730

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events