14-3-3 eta antibody (AA 30-110)

Catalog No. ABIN7224956

The Rabbit Polyclonal anti-14-3-3 eta antibody (ABIN7224956) specifically detects 14-3-3 eta in WB, ELISA, IF and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for 14-3-3 eta antibody (AA 30-110) (ABIN7224956)

Target: 14-3-3 eta (YWHAH)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 eta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-14-3-3 eta Antibody

Binding Specificity: AA 30-110

Purpose: Rabbit Anti-14-3-3 η Polyclonal Antibody

Specificity: 14-3-3 η Polyclonal Antibody detects endogenous levels of 14-3-3 η protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human 14-3-3 eta at AA range: 30-110

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for 14-3-3 eta

Target: 14-3-3 eta (YWHAH)

Alternative Name: 14-3-3 eta

Background: YWHAH, YWHA1, 14-3-3 protein eta, Protein AS1YWHAH (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta) product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse, rat and bovine orthologs. YWHAH contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder.

Molecular Weight: 42kD

Gene ID: 7533

UniProt: Q04917

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, C21-Steroid Hormone Metabolic Process, Myometrial Relaxation and Contraction