14-3-3 theta antibody

Catalog No. ABIN7213376

This Rabbit Polyclonal antibody specifically detects 14-3-3 theta in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for 14-3-3 theta antibody (ABIN7213376)

Target: 14-3-3 theta (YWHAQ)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 theta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-14-3-3 theta Antibody

Purpose: Rabbit Anti-14-3-3 θ/τ Polyclonal Antibody

Specificity: 14-3-3 θ/τ Polyclonal Antibody detects endogenous levels of 14-3-3 θ/τ protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human 14-3-3 theta/tau around the non-phosphorylation site of S232

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for 14-3-3 theta

Target: 14-3-3 theta (YWHAQ)

Alternative Name: 14-3-3 theta/tau

Background: YWHAQ, 14-3-3 protein theta, 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.

Molecular Weight: 28kD

Gene ID: 10971

UniProt: P27348

Pathways: Apoptosis, Myometrial Relaxation and Contraction