PDPK1 antibody (pSer241)

Catalog No. ABIN7223088

The Rabbit Polyclonal anti-PDPK1 antibody (ABIN7223088) specifically detects PDPK1 in WB, ELISA and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PDPK1 antibody (pSer241) (ABIN7223088)

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDPK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PDPK1 Antibody

Binding Specificity: pSer241

Purpose: PDK1 (phospho Ser241) Polyclonal Antibody

Specificity: Phospho-PDK1 (S241) Polyclonal Antibody detects endogenous levels of PDK1 protein only when phosphorylated at S241.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human PDK1 Phospho-Ser241

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PDPK1

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Alternative Name: PDK1

Background: Rabbit Anti-PDK1 (phospho Ser241) Polyclonal Antibody,PDPK1, PDK1, 3-phosphoinositide-dependent protein kinase 1, hPDK1,PDPK1 (3-Phosphoinositide Dependent Protein Kinase 1) is a Protein Coding gene. Among its related pathways are Aldosterone-regulated sodium reabsorption and AMPK signaling pathway. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity.,3-phosphoinositide-dependent protein kinase 1

Molecular Weight: observerd band 63kDa

Gene ID: 5170

UniProt: O15530

Pathways: PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals