CD73 antibody (Internal Region)

Catalog No. ABIN7218044

The Rabbit Polyclonal anti-CD73 antibody is suitable to detect CD73 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF and ELISA.

Quick Overview for CD73 antibody (Internal Region) (ABIN7218044)

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD73 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-CD73 Antibody

Binding Specificity: Internal Region

Purpose: Rabbit Anti-CD73 Polyclonal Antibody

Specificity: CD73 Polyclonal Antibody detects endogenous levels of CD73 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human CD73

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC: 1:100-300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: CD73

Background: NT5E, NT5, NTE, 5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, CD735'-nucleotidase ecto encoded by NT5E is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 68kD

Gene ID: 4907

UniProt: P21589

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process