AKAP1 antibody (AA 250-330)

Catalog No. ABIN7213524

The Rabbit Polyclonal anti-AKAP1 antibody (ABIN7213524) specifically detects AKAP1 in WB, IHC, IF and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for AKAP1 antibody (AA 250-330) (ABIN7213524)

Target: AKAP1 (A Kinase (PRKA) Anchor Protein 1 (AKAP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-AKAP1 Antibody

Binding Specificity: AA 250-330

Purpose: Rabbit Anti-AKAP 149 Polyclonal Antibody

Specificity: AKAP 149 Polyclonal Antibody detects endogenous levels of AKAP 149 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human AKAP 149 at AA range: 250-330

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for AKAP1

Target: AKAP1 (A Kinase (PRKA) Anchor Protein 1 (AKAP1))

Alternative Name: AKAP 149

Background: AKAP1, AKAP149, PRKA1, A-kinase anchor protein 1, mitochondrial, A-kinase anchor protein 149 kDa, AKAP 149, Dual specificity A-kinase-anchoring protein 1, D-AKAP-1, Protein kinase A-anchoring protein 1, PRKA1, Spermatid A-kinase anchor protThe A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. AKAP1 encodes a member of the AKAP family. The encoded protein (A-kinase anchoring protein 1) binds to type I and type II regulatory subunits of PKA and anchors them to the mitochondrion. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment.

Molecular Weight: 90kD

Gene ID: 8165

UniProt: Q92667

Pathways: Synaptic Membrane