AKAP14 antibody (AA 10-90)

Catalog No. ABIN7219936

The Rabbit Polyclonal anti-AKAP14 antibody is suitable to detect AKAP14 in samples from Human and Rat. It has been validated for WB, ELISA, IF and IHC.

Quick Overview for AKAP14 antibody (AA 10-90) (ABIN7219936)

Target: AKAP14 (A Kinase (PRKA) Anchor Protein 14 (AKAP14))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKAP14 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-AKAP14 Antibody

Binding Specificity: AA 10-90

Purpose: Rabbit Anti-AKAP 14 Polyclonal Antibody

Specificity: AKAP 14 Polyclonal Antibody detects endogenous levels of AKAP 14 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human AKAP 14 at AA range: 10-90

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for AKAP14

Target: AKAP14 (A Kinase (PRKA) Anchor Protein 14 (AKAP14))

Alternative Name: AKAP 14

Background: AKAP14, AKAP28, A-kinase anchor protein 14, AKAP-14, A-kinase anchor protein 28 kDa, AKAP 28, Protein kinase A-anchoring protein 14, PRKA14The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The protein anchors PKA in ciliary axonemes and, in this way, may play a role in regulating ciliary beat frequency. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Molecular Weight: 23kD

Gene ID: 158798

UniProt: Q86UN6