Actin, Alpha, gamma antibody

Catalog No. ABIN7223846

The Rabbit Polyclonal anti-Actin, Alpha, gamma antibody is suitable to detect Actin, Alpha, gamma in samples from Human, Mouse and Rat. It has been validated for IHC, ELISA, IF and WB.

Quick Overview for Actin, Alpha, gamma antibody (ABIN7223846)

Target: Actin, Alpha, gamma

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin, Alpha, gamma antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Western Blotting (WB)

Product Details anti-Actin, Alpha, gamma Antibody

Purpose: Rabbit Anti-Actin-α/γ Polyclonal Antibody

Specificity: Actin-α/γ Polyclonal Antibody detects endogenous levels of Actin-α/γ protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Actin-alpha/gamma around the non-phosphorylation site of Y55/53

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Actin, Alpha, gamma

Target: Actin, Alpha, gamma

Alternative Name: Actin-alpha/gamma

Background: ACTC1, ACTC, Actin, alpha cardiac muscle 1, Alpha-cardiac actin, ACTG1, ACTB, ACTG, Actin, cytoplasmic 2, Gamma-actin, ACTG2, ACTA3, ACTL3, ACTSG, Actin, gamma-enteric smooth muscle, Alpha-actin-3, Gamma-2-actin, Smooth muscle gamma-actin,The product encoded by ACTA1 (actin, alpha 1, skeletal muscle) belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in ACTA1 cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

Molecular Weight: 45kD

Gene ID: 58, 70, 71

UniProt: P68032, P63261, P63267, P68133