Actin, Alpha, gamma antibody (pTyr53, pTyr55)

Catalog No. ABIN7224640

The Rabbit Polyclonal anti-Actin, Alpha, gamma antibody (ABIN7224640) specifically detects Actin, Alpha, gamma in IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Actin, Alpha, gamma antibody (pTyr53, pTyr55) (ABIN7224640)

Target: Actin, Alpha, gamma

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin, Alpha, gamma antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Actin, Alpha, gamma Antibody

Binding Specificity: pTyr53, pTyr55

Purpose: Rabbit Anti-Actin-α/γ (phospho Tyr55/53) Polyclonal Antibody

Specificity: Phospho-Actin-α/γ (Y55/53) Polyclonal Antibody detects endogenous levels of Actin-α/γ protein only when phosphorylated at Y55/53.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Actin-alpha/gamma Phospho-Tyr55/53

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Actin, Alpha, gamma

Target: Actin, Alpha, gamma

Alternative Name: Actin-alpha/gamma

Background: ACTC1, ACTC, Actin, alpha cardiac muscle 1, Alpha-cardiac actin, ACTG1, ACTB, ACTG, Actin, cytoplasmic 2, Gamma-actin, ACTG2, ACTA3, ACTL3, ACTSG, Actin, gamma-enteric smooth muscle, Alpha-actin-3, Gamma-2-actin, Smooth muscle gamma-actin,Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by ACTC1 (actin, alpha, cardiac muscle 1) belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in ACTC1 have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC).

Gene ID: 70, 71, 72

UniProt: P68032, P63261, P63267, P68133