ACTN2/3 antibody (AA 1-80)

Catalog No. ABIN7213470

This anti-ACTN2/3 antibody is a Rabbit Polyclonal antibody detecting ACTN2/3 in WB, ELISA, IF and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for ACTN2/3 antibody (AA 1-80) (ABIN7213470)

Target: ACTN2/3 (Actinin, alpha 2/3 (ACTN2/3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTN2/3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ACTN2/3 Antibody

Binding Specificity: AA 1-80

Purpose: Actinin-α2/3 Polyclonal Antibody

Specificity: Actinin-α2/3 Polyclonal Antibody detects endogenous levels of Actinin-α2/3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the N-terminal region of human Actinin-alpha2/3 at AA range: 1-80

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for ACTN2/3

Target: ACTN2/3 (Actinin, alpha 2/3 (ACTN2/3))

Alternative Name: Actinin-alpha2/3

Background: Rabbit Anti-Actinin-α2/3 Polyclonal Antibody,ACTN2, Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2, F-actin cross-linking protein, ACTN3, Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, F-actin cross-linking protein,Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. ACTN2 encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for ACTN2.,Alpha-actinin-2

Gene ID: 88, 89