ACTN3 antibody (AA 10-90)

Catalog No. ABIN7213472

This Rabbit Polyclonal antibody specifically detects ACTN3 in WB, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ACTN3 antibody (AA 10-90) (ABIN7213472)

Target: ACTN3 (Actinin, alpha 3 (ACTN3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTN3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-ACTN3 Antibody

Binding Specificity: AA 10-90

Purpose: Actinin-α3 Polyclonal Antibody

Specificity: Actinin-α3 Polyclonal Antibody detects endogenous levels of Actinin-α3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the N-terminal region of human Actinin-alpha3 at AA range: 10-90

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for ACTN3

Target: ACTN3 (Actinin, alpha 3 (ACTN3))

Alternative Name: Actinin-alpha3

Background: Rabbit Anti-Actinin-α3 Polyclonal Antibody,ACTN3, Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, F-actin cross-linking protein,ACTN3 encodes a member of the alpha-actin binding protein gene family. Actinin alpha 3 (gene/pseudogene) is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants, the reference genome represents the coding allele. The non-functional allele of ACTN3 is associated with elite athlete status.,Alpha-actinin-3

Gene ID: 89

UniProt: Q08043

Pathways: Cell-Cell Junction Organization