AFT1 antibody

Catalog No. ABIN7222640

The Rabbit Polyclonal anti-AFT1 antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect AFT1 in samples from Human and Mouse.

Quick Overview for AFT1 antibody (ABIN7222640)

Target: AFT1 (Activating Transcription Factor 1 (AFT1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AFT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-AFT1 Antibody

Purpose: ATF-1 Polyclonal Antibody

Specificity: ATF-1 Polyclonal Antibody detects endogenous levels of ATF-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human ATF-1 around the non-phosphorylation site of S63

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for AFT1

Target: AFT1 (Activating Transcription Factor 1 (AFT1))

Alternative Name: ATF-1

Background: Rabbit Anti-ATF-1 Polyclonal Antibody,ATF1, Cyclic AMP-dependent transcription factor ATF-1, cAMP-dependent transcription factor ATF-1, Activating transcription factor 1, Protein TREB36,ATF1 encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of ATF1 and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. ATF1 has a pseudogene on chromosome 6.,Cyclic AMP-dependent transcription factor ATF-1

Gene ID: 466

UniProt: P18846

Pathways: Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades