ATF2 antibody (pSer112)

Catalog No. ABIN7212876

This anti-ATF2 antibody is a Rabbit Polyclonal antibody detecting ATF2 in WB, ELISA, IP and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for ATF2 antibody (pSer112) (ABIN7212876)

Target: ATF2 (Activating Transcription Factor 2 (ATF2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ATF2 Antibody

Binding Specificity: pSer112

Purpose: ATF-2 (phospho Ser112) Polyclonal Antibody

Specificity: Phospho-ATF-2 (S112) Polyclonal Antibody detects endogenous levels of ATF-2 protein only when phosphorylated at S112.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human ATF-2 Phospho-Ser112

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IP (2-5 μg/mg lysate), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for ATF2

Target: ATF2 (Activating Transcription Factor 2 (ATF2))

Alternative Name: ATF-2

Background: Rabbit Anti-ATF-2 (phospho Ser112) Polyclonal Antibody,ATF2, CREB2, CREBP1, Cyclic AMP-dependent transcription factor ATF-2, cAMP-dependent transcription factor ATF-2, Activating transcription factor 2, Cyclic AMP-responsive element-binding protein 2, CREB-2, cAMP-responsive element-binding pro,ATF2 encodes a transcription factor (activating transcription factor 2) that is a member of the leucine zipper family of DNA binding proteins. Activating transcription factor 2 has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Activating transcription factor 2 binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro, thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Activating transcription factor 2 may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this ATF2.,Cyclic AMP-dependent transcription factor ATF-2

Gene ID: 1386

UniProt: P15336

Pathways: MAPK Signaling, RTK Signaling, Thyroid Hormone Synthesis, Activation of Innate immune Response, Chromatin Binding, Myometrial Relaxation and Contraction, Synaptic Membrane, Tube Formation, Toll-Like Receptors Cascades