ATF5 antibody (acLys29)

Catalog No. ABIN7212588

This Rabbit Polyclonal antibody specifically detects ATF5 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ATF5 antibody (acLys29) (ABIN7212588)

Target: ATF5 (Activating Transcription Factor 5 (ATF5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATF5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ATF5 Antibody

Binding Specificity: acLys29

Purpose: Rabbit Anti-ATF-5 (Acetyl Lys29) Polyclonal Antibody

Specificity: Acetyl-ATF-5 (K29) Polyclonal Antibody detects endogenous levels of ATF-5 protein only when acetylated at K29.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the human ATF-5 around the acetylation site of K29

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ATF5

Target: ATF5 (Activating Transcription Factor 5 (ATF5))

Alternative Name: ATF-5

Background: ATF5, ATFX, Cyclic AMP-dependent transcription factor ATF-5, cAMP-dependent transcription factor ATF-5, Activating transcription factor 5, Transcription factor ATFxATF5 or Activating transcription factor 5, binds to cAMP inducible promoters and is involved in gene transcription. Activating transcription factor 5 binds the cAMP response element (CRE)(consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. ATF5 plays a role in inhibition of nerve growth factor induced neuronal outgrowth and regulation of neurogenesis.

Molecular Weight: 30kD

Gene ID: 22809

UniProt: Q9Y2D1

Pathways: Myometrial Relaxation and Contraction