ATF5 antibody (AA 190-270)

Catalog No. ABIN7213714

The Rabbit Polyclonal anti-ATF5 antibody is suitable to detect ATF5 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for ATF5 antibody (AA 190-270) (ABIN7213714)

Target: ATF5 (Activating Transcription Factor 5 (ATF5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATF5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-ATF5 Antibody

Binding Specificity: AA 190-270

Purpose: Rabbit Anti-ATF-5 Polyclonal Antibody

Specificity: ATF-5 Polyclonal Antibody detects endogenous levels of ATF-5 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human ATF-5 at AA range: 190-270

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ATF5

Target: ATF5 (Activating Transcription Factor 5 (ATF5))

Alternative Name: ATF-5

Background: ATF5, ATFX, Cyclic AMP-dependent transcription factor ATF-5, cAMP-dependent transcription factor ATF-5, Activating transcription factor 5, Transcription factor ATFxEukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The best characterized members of this gene family include CREB-1, CREB-2 (also designated ATF-4), CRE-BPa, LZIP (also designated CREB3 and Luman), CREM-1, CREM-2, ATF-1, ATF-2, ATF-3, ATF-5, ATF-6 and ATF-7. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-5 (ATFx), which can localize to the cytoplasm or the nucleus, binds DNA as a dimer. It interacts with CCND3 and PTP4A1.

Molecular Weight: 31kD

Gene ID: 22809

UniProt: Q9Y2D1

Pathways: Myometrial Relaxation and Contraction