ADAM17 antibody (pThr735)

Catalog No. ABIN7224582

The Rabbit Polyclonal anti-ADAM17 antibody (ABIN7224582) specifically detects ADAM17 in WB, ELISA and IHC (p). The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for ADAM17 antibody (pThr735) (ABIN7224582)

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADAM17 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ADAM17 Antibody

Binding Specificity: pThr735

Purpose: TACE (phospho Thr735) Polyclonal Antibody

Specificity: Phospho-TACE (T735) Polyclonal Antibody detects endogenous levels of TACE protein only when phosphorylated at T735.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human TACE Phospho-Thr735

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for ADAM17

Target: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Alternative Name: TACE

Background: Rabbit Anti-TACE (phospho Thr735) Polyclonal Antibody,ADAM17, CSVP, TACE, Disintegrin and metalloproteinase domain-containing protein 17, ADAM 17, Snake venom-like protease, TNF-alpha convertase, TNF-alpha-converting enzyme, CD antigen CD156b,ADAM17 (ADAM metallopeptidase domain 17) encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The encoded preproprotein is proteolytically processed to generate the mature protease. The encoded protease functions in the ectodomain shedding of tumor necrosis factor-alpha, in which soluble tumor necrosis factor-alpha is released from the membrane-bound precursor. This protease also functions in the processing of numerous other substrates, including cell adhesion proteins, cytokine and growth factor receptors and epidermal growth factor (EGF) receptor ligands. The encoded protein also plays a prominent role in the activation of the Notch signaling pathway. Elevated expression of ADAM17 has been observed in specific cell types derived from psoriasis, rheumatoid arthritis, multiple sclerosis and Crohn's disease patients, suggesting that the encoded protein may play a role in autoimmune disease.,Disintegrin and metalloproteinase domain-containing protein 17

Molecular Weight: observerd band 93kDa

Gene ID: 6868

UniProt: P78536

Pathways: Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus