alpha Adducin antibody (pThr445)

Catalog No. ABIN7219384

The Rabbit Polyclonal anti-alpha Adducin antibody is suitable to detect alpha Adducin in samples from Human, Mouse and Rat. It has been validated for IHC, ELISA and IF.

Quick Overview for alpha Adducin antibody (pThr445) (ABIN7219384)

Target: alpha Adducin (ADD1) (Adducin 1 (Alpha) (ADD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Adducin antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-alpha Adducin Antibody

Binding Specificity: pThr445

Purpose: Rabbit Anti-Adducin α (phospho Thr445) Polyclonal Antibody

Specificity: Phospho-Adducin α (T445) Polyclonal Antibody detects endogenous levels of Adducin α protein only when phosphorylated at T445.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Adducin alpha Phospho-Thr445

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for alpha Adducin

Target: alpha Adducin (ADD1) (Adducin 1 (Alpha) (ADD1))

Alternative Name: Adducin alpha

Background: ADD1, ADDA, Alpha-adducin, Erythrocyte adducin subunit alphaAdducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms, however, not all variants have been fully described.

Gene ID: 118

UniProt: P35611

Pathways: Negative Regulation of Hormone Secretion, Regulation of Actin Filament Polymerization, Regulation of Lipid Metabolism by PPARalpha, ER-Nucleus Signaling