AFF4 antibody (AA 30-110)

Catalog No. ABIN7227230

The Rabbit Polyclonal anti-AFF4 antibody is suitable to detect AFF4 in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for AFF4 antibody (AA 30-110) (ABIN7227230)

Target: AFF4 (AF4/FMR2 Family, Member 4 (AFF4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AFF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-AFF4 Antibody

Binding Specificity: AA 30-110

Purpose: Rabbit Anti-AFF4 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of AFF4 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human AFF4 protein at AA range: 30-110

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for AFF4

Target: AFF4 (AF4/FMR2 Family, Member 4 (AFF4))

Alternative Name: AFF4

Background: AF4/FMR2 family member 4, ALL1-fused gene from chromosome 5q31 protein, Protein AF-5q31, Major CDK9 elongation factor-associated proteinAFF4 (AF4/FMR2 Family Member 4) is a Protein Coding gene. Diseases associated with AFF4 include Chops Syndrome and Lymphoblastic Leukemia. Among its related pathways are Formation of HIV elongation complex in the absence of HIV Tat and Gene Expression. The protein encoded by AFF4 belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. A chromosomal translocation involving AFF4 and MLL gene on chromosome 11 is found in infant acute lymphoblastic leukemia with ins.

Molecular Weight: 127kD

Gene ID: 27125

UniProt: Q9UHB7