Aggrecan antibody (Cleaved-Asp369)

Catalog No. ABIN7220062

This Rabbit Polyclonal antibody specifically detects Aggrecan in WB, IHC, ELISA and IF. It exhibits reactivity toward Human and Rat.

Quick Overview for Aggrecan antibody (Cleaved-Asp369) (ABIN7220062)

Target: Aggrecan (ACAN)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aggrecan antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Aggrecan Antibody

Binding Specificity: AA 290-370, Cleaved-Asp369

Purpose: Rabbit Anti-Cleaved-Aggrecan (D369) Polyclonal Antibody

Specificity: Cleaved-Aggrecan (D369) Polyclonal Antibody detects endogenous levels of fragment of activated Aggrecan protein resulting from cleavage adjacent to D369.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human Aggrecan at AA range: 290-370

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Aggrecan

Target: Aggrecan (ACAN)

Alternative Name: Aggrecan

Background: ACAN, AGC1, CSPG1, MSK16, Aggrecan core protein, Cartilage-specific proteoglycan core protein, CSPCP, Chondroitin sulfate proteoglycan core protein 1, Chondroitin sulfate proteoglycan 1ACAN is a member of the aggrecan/versican proteoglycan family. Aggrecan core protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene.

Molecular Weight: 44kD

Gene ID: 176

UniProt: P16112

Pathways: Glycosaminoglycan Metabolic Process, Dicarboxylic Acid Transport