AGRN antibody (AA 1900-1980)

Catalog No. ABIN7227240

The Rabbit Polyclonal anti-AGRN antibody has been validated for WB and ELISA. It is suitable to detect AGRN in samples from Human, Rat and Mouse.

Quick Overview for AGRN antibody (AA 1900-1980) (ABIN7227240)

Target: AGRN (Agrin (AGRN))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGRN antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-AGRN Antibody

Binding Specificity: AA 1900-1980

Purpose: AGRIN Polyclonal Antibody

Specificity: The antibody detects endogenous levels of AGRIN protein

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen

Immunogen: Synthesized peptide derived from part region of human AGRIN protein at AA range: 1900-1980

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000-1:20000).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for AGRN

Target: AGRN (Agrin (AGRN))

Alternative Name: AGRIN

Background: Rabbit Anti-AGRIN Polyclonal Antibody,Agrin, Agrin N-terminal 110 kDa subunit, Agrin C-terminal 110 kDa subunit, Agrin C-terminal 90 kDa fragment, C90, Agrin C-terminal 22 kDa fragment, C22,AGRN encodes one of several proteins that are critical in the development of the neuromuscular junction (NMJ), as identified in mouse knock-out studies. The encoded protein contains several laminin G, Kazal type serine protease inhibitor, and epidermal growth factor domains. Additional post-translational modifications occur to add glycosaminoglycans and disulfide bonds. In one family with congenital myasthenic syndrome affecting limb-girdle muscles, a mutation in AGRN was found. Alternative splicing results in multiple transcript variants encoding different isoforms.,AGRIN

Gene ID: 375790

UniProt: O00468

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development