AKT1/3 (pTyr434), (pTyr437) antibody

Catalog No. ABIN7213280

This anti-not_set antibody is a Rabbit Polyclonal antibody detecting not_set in ELISA, WB and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for AKT1/3 (pTyr434), (pTyr437) antibody (ABIN7213280)

Target: AKT1/3

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: pTyr434, pTyr437

Purpose: Akt1/3 (phospho Tyr437/434) Polyclonal Antibody

Specificity: Phospho-Akt1/3 (Y437/434) Polyclonal Antibody detects endogenous levels of Akt1/3 protein only when phosphorylated at Y437/434.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Akt1/3 Phospho-Tyr437/434

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details

Target: AKT1/3

Background: Rabbit Anti-Akt1/3 (phospho Tyr437/434) Polyclonal Antibody,AKT1, PKB, RAC, RAC-alpha serine/threonine-protein kinase, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT3, PKBG, RAC-gamma serine/threonine-protein kinase, Protein kinase Akt-3, Protein ki,The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in AKT1 have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for AKT1.,RAC-alpha serine threonine-protein kinase

Gene ID: 207, 10000